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  • Required metadata values are missing - Cuffdiff error

    I am trying to perform differential expression analysis of two conditions on bam files from 50bp, SE sequenced mRNA-seq constructed libraries.

    I am using mm9 as reference genome (UCSC)

    Everytime I start Cuffdiff on Galaxy I get the following error report.

    "Required metadata values are missing. Some of these values may not be editable by the user. Selecting "Auto-detect" will attempt to fix these values."

    Any suggestions how to fix this problem would be greatly appreciated!!

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