Hi all,
We just got some bisulfite sequencing data from a set of patients. After running FastQC on a few samples, we observed an extremely low quality score at position #51, for two samples.
Attached are the distribution of base quality scores, % of Ns, and the nucleotide composition at each position.
My question is, what is the possible reason for this? Should we trim all the nucleotides after 50?
Thanks in advance!
We just got some bisulfite sequencing data from a set of patients. After running FastQC on a few samples, we observed an extremely low quality score at position #51, for two samples.
Attached are the distribution of base quality scores, % of Ns, and the nucleotide composition at each position.
My question is, what is the possible reason for this? Should we trim all the nucleotides after 50?
Thanks in advance!
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