Complete Newbie, and a current undergrad. I'm still trying to grip this entire field along with the vocabulary associated with it. So if my questions are a bit convoluted--I really appreciate your patience!
This is purely hypothetical, but....
....I'm sequencing a whole human genome from Saliva.
After taking the saliva sample the next step is sample prep (correct?)
Sample prep includes DNA amplification and purification (correct?)
After that, what are the samples loaded onto? I read that certain companies process 96 well plates (http://www.operon.com/services/dna-s...rice-list.aspx)
...so does that mean one sample per well? Or does one sample get divided into all 96 wells?
Then they're run through next gen sequencers? (Is there another process in between?)
After they're run through the sequencers, they go through bioinformatics processing. I get a fastq file with a bunch of short reads that have to be assembled (de novo or from a reference). [I have a pretty decent understanding of this area].
Or am I missing a whole lot? As you can see, it's really difficult for me to complete a picture without understanding the technical vocabulary.
This is purely hypothetical, but....
....I'm sequencing a whole human genome from Saliva.
After taking the saliva sample the next step is sample prep (correct?)
Sample prep includes DNA amplification and purification (correct?)
After that, what are the samples loaded onto? I read that certain companies process 96 well plates (http://www.operon.com/services/dna-s...rice-list.aspx)
...so does that mean one sample per well? Or does one sample get divided into all 96 wells?
Then they're run through next gen sequencers? (Is there another process in between?)
After they're run through the sequencers, they go through bioinformatics processing. I get a fastq file with a bunch of short reads that have to be assembled (de novo or from a reference). [I have a pretty decent understanding of this area].
Or am I missing a whole lot? As you can see, it's really difficult for me to complete a picture without understanding the technical vocabulary.
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