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I just tried to determine miRNA features, I computed most but I still don't know how to compute the structure features like the stem length, base paring probability, largest bulge, gap ratio,,etc I tried to get my answer with the Vienna package but I couldn't. What do you suggest. Actually, I tried to extract all the possible features of the secondary structure of miRNA, I used perl and Bioperl but I didn't find any function or package to help me to get the stem length, base paring probability, largest bulge, gap ratio..etc
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM