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  • Weird pileups

    Hi

    In some genes (e.g. PRKRA and POLR2C) we sometimes see pileups from our exome-data like this (nimblegen medexome, illumina NextSeq 2x75bp). It almost look like we have spiked in cDNA into our genomic DNA sample ? Can it be pseudo genes ?

    Anybody who have seen this phenomenon before ?



    Best,
    Martin
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