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I have identified frequency of SNPs, heterozygotes and homozygotes per 100kb for my eukaryotic species (which is maintained for 100 generations by inbreeding) by readmapping illumina paired-reads to reference genome. From Drake et al. Genetics, 1998, it is inferred that fixed mutations for eurkayotes (mutation rate are measured in substitutions) is 0.003 mutations per genome per generation. Can I use this for mutation rate calculation for of my eurkaryotic species?. How can I correlate my mutation rate to zygosity?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM