Hi all,
Bowtie's manual says the following about -v mode:
"-v <int> Report alignments with at most <int> mismatches"
I understood from this that for paired end reads the whole alignment, including both ends, will contain up to <int> mismatches.
But when I ran my data with -v 2 mode I got the following MD:Z values for 2 ends of the same read:
23A3A36
59T3T0
and another example:
32A13G17
15A21A26
So it seems like 2 mismatches are allowed per end.
Just wanted to make sure that this what -v means, and that I didn't do something else that caused this.
Thanks,
Rachelly.
Bowtie's manual says the following about -v mode:
"-v <int> Report alignments with at most <int> mismatches"
I understood from this that for paired end reads the whole alignment, including both ends, will contain up to <int> mismatches.
But when I ran my data with -v 2 mode I got the following MD:Z values for 2 ends of the same read:
23A3A36
59T3T0
and another example:
32A13G17
15A21A26
So it seems like 2 mismatches are allowed per end.
Just wanted to make sure that this what -v means, and that I didn't do something else that caused this.
Thanks,
Rachelly.