Hi there!
I'm new to the world of library prep for MiSeq thought I would reach out to the community for some suggestions. We are preparing 16S libraries from low density microbial communities with primers designed for the V1/V3 hypervariable region of 16s rRNA followed by barcoding. We are having problems eliminating the adapter artifact peak ~130 bp (usually 4x greater concentration than our 300 bp product) even after bead size selection. Anybody know any tricks for eliminating the artifacts?
I'm new to the world of library prep for MiSeq thought I would reach out to the community for some suggestions. We are preparing 16S libraries from low density microbial communities with primers designed for the V1/V3 hypervariable region of 16s rRNA followed by barcoding. We are having problems eliminating the adapter artifact peak ~130 bp (usually 4x greater concentration than our 300 bp product) even after bead size selection. Anybody know any tricks for eliminating the artifacts?
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