Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Extracting REF and ALT from VCF

    This may be a simple question.

    I am trying to extract CHR, POS, REF and ALT and other info from a VCF file to a txt file using vcftools. Can somebody point me how to get REF and ALT? I don't get this information but everything else.

    Thanks
    -K.

  • #2
    I think vcf-to-tab does exactly that:
    vcf-to-tab < file.vcf

    Comment


    • #3
      Originally posted by id0 View Post
      I think vcf-to-tab does exactly that:
      vcf-to-tab < file.vcf
      Thank you, id0. It works and I get the genotypes. But I don't get other information from the FORMAT fields. I know I can use --extract-FORMAT-info separately and 'paste' these two lists, but I would be more comfortable if I can get the genotypes as well as the format fields information at the same time. Anyway, I thank you for the solution.

      Comment


      • #4
        Originally posted by kasthuri View Post
        Thank you, id0. It works and I get the genotypes. But I don't get other information from the FORMAT fields. I know I can use --extract-FORMAT-info separately and 'paste' these two lists, but I would be more comfortable if I can get the genotypes as well as the format fields information at the same time. Anyway, I thank you for the solution.
        Also, I will be just happy with the ALT entries instead of actual variants. Is it possible to get that?

        Thanks
        -K.

        Comment


        • #5
          vcf's are naturally text files. If yours isn't, it's just compressed, so uncompress it.

          Comment


          • #6
            Originally posted by swbarnes2 View Post
            vcf's are naturally text files. If yours isn't, it's just compressed, so uncompress it.
            Thank you swbarnes2. I found VariantsToTable in GATK does exactly what I wanted.

            Comment

            Latest Articles

            Collapse

            • seqadmin
              Essential Discoveries and Tools in Epitranscriptomics
              by seqadmin


              The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
              Yesterday, 07:01 AM
            • seqadmin
              Current Approaches to Protein Sequencing
              by seqadmin


              Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
              04-04-2024, 04:25 PM

            ad_right_rmr

            Collapse

            News

            Collapse

            Topics Statistics Last Post
            Started by seqadmin, 04-11-2024, 12:08 PM
            0 responses
            39 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 04-10-2024, 10:19 PM
            0 responses
            41 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 04-10-2024, 09:21 AM
            0 responses
            35 views
            0 likes
            Last Post seqadmin  
            Started by seqadmin, 04-04-2024, 09:00 AM
            0 responses
            55 views
            0 likes
            Last Post seqadmin  
            Working...
            X