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  • Intro post

    Hey all,

    I'm Kim Ferguson, and I'm a PhD researcher at Wageningen University in The Netherlands, though I'm originally from Canada and completed my MSc Ecology at the University of Bremen in Germany.

    Now, as I mentioned I have a background in ecology (behavioural and population ecology to be more precise), and little-to-no molecular biology experience, so switching to a project that aims to do whole genome assembly and analysis on arthropod biocontrol species is quite a leap, and I'm feeling the difference in methodlogies and mentalities to be... interesting? stressful? We'll see, but essentially I'm doing a lot of knowledge gap-filling at the moment.

    The first obstacle is picking a sequencing method and subsequent assembly method, so I hope to gain some insight from the SeqAnswers reasources Where did I hear about this site? A few different resources, but definitely Ekblom and Wolf's 2014 article, "A field guide to whole-genome sequencing, assembly and annotation" played a part.

    So. If you're interested, my project is RP1 of the BINGO-ITN: Breeding Invertebrates for Next Generation BioControl, an Innovative Training Network. I'll be working with Amblyseius swirskii, Nesidiocoris tenuis, and Trichogramma brassicae - you can go to www.bingo-itn.eu for more info.

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  • seqadmin
    Current Approaches to Protein Sequencing
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    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
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    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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