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  • Maximum number of cycles for kit Miseq 50 cycles

    Hi sequencing Masters!

    What is the maximum number of cycles we can do with a Miseq V2 50 cycles kit. I have been told that there is enough reagents for more than 55 cycles including the 6 cycles for index reading. Can someone confirm this information?
    Thank you very much for your support. It is very appreciated.

  • #2
    How many are you looking to get?

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    • #3
      54 maximum + 6 for index

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      • #4
        It should be fine. Local core does 66 cycle (50x8x8) runs off and on.

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        • #5
          Great news. Thank you for your answer Genomax!

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          • #6
            I got an answer from Illumina's tech support:
            MiSeq v2 contains enough reagents for 75 cycles of chemistry

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            • #8
              I know this is late but we routinely do 78 cycles with the MiSeq v2 50-cycle kit. (31 read1, 31 read2, 8 index1, 8 index2).

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              • #9
                Plus 7 dark cycles used for index2 read so 85 cycles in total. This seems to be 10 cycles more than Illumina specification.
                Last edited by nucacidhunter; 10-18-2017, 09:30 PM.

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