Hi all,
I just prepared a NexteraXT library of mitochondrial DNA for a collaborator. Although they purified the mitochondrial DNA (removed genomic DNA), the sequencing results from the MiSeq suggest that it is still almost completely genomic DNA contaminants.
Does anybody have experience performing shotgun sequencing with mitochondrial DNA or, even better, using NexteraXT with the mito DNA? I figure one possibility is that the NexteraXT is not able to properly 'tagment' the mitochondrial DNA and is only processing the genomic DNA. The other possibility is that the samples are not pure enough.
If anybody could offer insight into preparing mitochondrial DNA libraries with NexteraXT, that would be amazing!
I just prepared a NexteraXT library of mitochondrial DNA for a collaborator. Although they purified the mitochondrial DNA (removed genomic DNA), the sequencing results from the MiSeq suggest that it is still almost completely genomic DNA contaminants.
Does anybody have experience performing shotgun sequencing with mitochondrial DNA or, even better, using NexteraXT with the mito DNA? I figure one possibility is that the NexteraXT is not able to properly 'tagment' the mitochondrial DNA and is only processing the genomic DNA. The other possibility is that the samples are not pure enough.
If anybody could offer insight into preparing mitochondrial DNA libraries with NexteraXT, that would be amazing!
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