Hi all, I am a complete novice at CLC genomics, and bioinformatics in general, and, with an absent PI, I am looking to the seqanswers community for help. Is anyone out there proficient enough with CLC genomics workbench to help me? I have done a batch style basic variant detection (0% threshold) of 17 E. coli genomes, all against a single reference genome, so now I have a single track with all the variants detected across all genomes. Next I want to annotate this track against a reference genome to identify more highly variable regions. I have already contacted CLC support, but am hoping to get a faster response on here. I hope this question makes sense.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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