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Old 09-10-2010, 12:08 AM   #1
genelab
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Default Using reference sequence in Velvet

hello all,
We have sequenced one kind of strain of a microorganism and get the pair-end short reads data. Then we want to use Velvet to assembly the genome sequence and use the genome sequence of other kind of stain of this microorganism as the reference sequence. Can someone tell me how to do that.
Any suggestion will be very appreciate!
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Old 09-10-2010, 01:07 AM   #2
jts
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You may wish to try the recently-released "Columbus" module for velvet. You can find the manual for it on the velvet webpage here:

http://www.ebi.ac.uk/~zerbino/velvet/
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Old 12-02-2013, 02:29 AM   #3
arkilis
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Quote:
Originally Posted by jts View Post
You may wish to try the recently-released "Columbus" module for velvet. You can find the manual for it on the velvet webpage here:

http://www.ebi.ac.uk/~zerbino/velvet/
I got the same issue as well, but cant find the Columbus you mentioned.. Might tried other pipelines...
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