Hi everyone,
I'm new to next-gen sequencing. Our library is a PCR product and I will have to design primers that include the adapter sequences. I"m still trying to get oriented and was wondering whether anyone has any into to sequencing reviews or literature (specifically Illumina/Solexa is what we're planning on using) and own primer design recommendations. (The Solexa website wasn' t specific enough and the Nature methods Jan 2008 primer was a good start, but I need a lot more detail to actually design the primers).
Thanks so much,
D
I'm new to next-gen sequencing. Our library is a PCR product and I will have to design primers that include the adapter sequences. I"m still trying to get oriented and was wondering whether anyone has any into to sequencing reviews or literature (specifically Illumina/Solexa is what we're planning on using) and own primer design recommendations. (The Solexa website wasn' t specific enough and the Nature methods Jan 2008 primer was a good start, but I need a lot more detail to actually design the primers).
Thanks so much,
D
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