Hi,
I have illumina pipeline 1.3 RNA-seq paired-end data,I want to use bwa on galaxy.
The bwa on galaxy said it Must have Sanger-scaled quality values with ASCII offset 33 .
I know Illumina Pipeline 1.3 and 1.4. Using a Phred scale using ASCII 64 to 104,I don't understand can I run it ?
I am confused with Phred scale using ASCII 64 to 104 and ASCII offset 33
I have illumina pipeline 1.3 RNA-seq paired-end data,I want to use bwa on galaxy.
The bwa on galaxy said it Must have Sanger-scaled quality values with ASCII offset 33 .
I know Illumina Pipeline 1.3 and 1.4. Using a Phred scale using ASCII 64 to 104,I don't understand can I run it ?
I am confused with Phred scale using ASCII 64 to 104 and ASCII offset 33
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