Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • CEGMA : Genewise Error

    Hi all,

    I am trying to run CEGMA and I am getting the following error message that has me lost:
    Warning Error
    Unable to open gene.stat as gene stats file
    Warning Error
    Could not read gene statistics in gene.stat
    Warning Error
    Could not open BLOSUM62.bla as a filename for read Blast matrix
    Warning Error
    Could not open file codon.table as codon table file
    Warning Error
    Could not read codon table file in codon.table
    Fatal Error
    Could not build objects!

    Any help would be appreciated.
    Thanks

  • #2
    Can you please post the full output of CEGMA, including the command that you ran?

    Also...do you have the latest versions of CEGMA, GeneWise, BLAST+ etc.

    Regards,

    Keith

    Comment


    • #3
      Hi,

      I know this is a bit late but I just stumbled over this error today so I thought I'd make an account and post the solution.

      In my case, the environment variable WISECONFIGDIR was set incorrectly.

      I "fixed" that by downloading my own installation of wise, since genewise was already installed system-wide, from here : http://korflab.ucdavis.edu/Datasets/...2.3-rc7.tar.gz

      Then extracted it in my home directory, and set WISECONFIGDIR to the wisecfg/ subfolder of the extracted folder:

      export WISECONFIGDIR=/home/bla/wise2.2.3-rc7/wisecfg

      Now CEGMA works. This uses of course the system-wide installation of genewise.

      (As a sidenote: You can save some time by running CEGMA with the -ext flag, that way CEGMA will continue roughly from where it crashed - but might continue on incomplete results!)
      Last edited by philipp.bayer; 05-01-2014, 03:13 AM.

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin


        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
        Yesterday, 07:01 AM
      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      39 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      41 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      35 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      55 views
      0 likes
      Last Post seqadmin  
      Working...
      X