Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Nextera vs Nextera XT: what´s the difference? Simone78 Sample Prep / Library Generation 33 04-20-2017 05:25 AM
Using Nextera Indexes with Nextera XT Kit Angus's Microbiota Illumina/Solexa 7 11-11-2016 11:06 PM
Choosing between Nextera and Nextera XT MMarti Sample Prep / Library Generation 1 11-10-2013 05:33 PM
Nextera XT gillt Sample Prep / Library Generation 0 03-06-2013 04:05 PM
nextera niceday Sample Prep / Library Generation 5 09-10-2010 10:31 AM

Thread Tools
Old 07-15-2015, 11:50 AM   #1
Location: Canada

Join Date: Jan 2014
Posts: 17
Default Squeezing the most out of Nextera XT...?

Hi All,

I'm wondering if anyone has experimented with pushing the limits of Nextera XT in order to get more samples out of a kit? I want to sequence a bunch (~150) of mitogenomes generated via long-range PCR. I was hoping to find a way to stretch the Nextera XT kit (and indices) to get the most bang for my buck. Since the samples are amplicons I think I should have enough "room" on one MiSeq run to collect all 150 mitogenomes at high depth...but don't want to have to pay for 150 Nextera XT libraries. Any ideas? Can I just run smaller reactions of the Nextera XT sample prep kit?

Thanks for any ideas.
SDK is offline   Reply With Quote
Old 07-16-2015, 07:11 AM   #2
Location: Cambridge, MA

Join Date: Apr 2011
Posts: 18

Have a look at this paper:
alec is offline   Reply With Quote

amplicon, illumina, miseq, mitogenome, nextera

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 06:01 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO