Hi,
I recently ordered sequencing of two libraries (mate pair and paired end) with an Illumina MiSeq (reads of 301 bp). Both libraries contain high amounts of T and C after base 250 in forward and reverse reads (picture attached).
To my understanding this is due to sequencing errors and I should trim of all bases after position 250?
Thank you for your advise
I recently ordered sequencing of two libraries (mate pair and paired end) with an Illumina MiSeq (reads of 301 bp). Both libraries contain high amounts of T and C after base 250 in forward and reverse reads (picture attached).
To my understanding this is due to sequencing errors and I should trim of all bases after position 250?
Thank you for your advise
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