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Old 01-24-2012, 07:19 AM   #1
JPC
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Default 5500 Wildfire

Not as exciting as the Proton news but LifeTech have put out some brief info on the 5500 development roadmap.

http://find.lifetechnologies.com/5500wildfire/ngse-pod

- 2 to 5 fold increase in density
- reduced sample prep time
- improved reverse reads
- forward read length may increase towards 100bp

has anyone heard any other info on this?

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Old 01-24-2012, 07:41 AM   #2
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I have heard it mentioned. It is similar to Bridge PCR, but different enough that ABI thinks it can win any infringement battles that Illumina launches against them. I would say the main issue is that this is little more than "vaporware" in all likelihood. My guess is that we will be looking at the same advertisement 1 year from now, similar to the timescales involved with roll out of the FLX+ technology from Roche.

That said, it would be nice to move away from the bead work. If ABI pushed, I am sure they could launch the product in a timely manner. But will they?

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Old 01-24-2012, 08:00 AM   #3
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So what does this mean for nanobeads ? Will this technology replace them ?
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Old 01-24-2012, 08:05 AM   #4
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I would assume so, this new system replaces the entire EZ bead prep, no more emPCR.

Last edited by JPC; 01-24-2012 at 08:18 AM.
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Old 01-24-2012, 09:31 AM   #5
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Anyone going to AGBT? According to the webinar there was going to be info about the release timeline updated there.

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Old 04-19-2012, 07:50 AM   #6
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I've just been told by AB that here in the UK the WildFire upgrade will be available in July and cost £70K. That will give you 16Gb/day output but it won't do paired ends reads until Q4 when the output will go up to 20Gb/day.

As run times will be increased they will limit you to 3 lanes per flow cell and they are suggesting a cost of US$25/Gb
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Old 05-30-2012, 10:26 AM   #7
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I heard something similar from the Lifetech France (although they are saying Sept for release). 69K euros, with specs along the lines mentioned by JPC. 5500 server needs to be updated (maybe included in the upgrade price?). I'd love to hear any comments re perforamce if there are any early access users out there???
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Old 05-30-2012, 01:56 PM   #8
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I think it'd be cooler if they offered an option between 3 or 6 lanes as different upgrade options. Something like a 5500 Wildfire (tm) and a 5500 Wildfire XL (tm). Who cares if the computer is bigger, the S4 had a cluster under it. I'd rather load all the projects on 12 lanes, then go on vacation while it runs for a month.

Who's got early access? Anyone know?
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Old 06-01-2012, 10:15 AM   #9
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http://ir.lifetechnologies.com/relea...leaseID=678584
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Old 07-24-2012, 10:32 AM   #10
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I spoke with an early adopter, they had 2 5500xl machines and updated 1 of them to wildfire and now no one uses the old one anymore and they can't wait to get the next one. Quality was as good as before, it runs faster and it is less work. So I'm really looking forward to playing with it myself. Sorry I don't have real data of any sorts.

I think he said the three lanes offer the same output as do 6 lanes now so overall it is the same. Biggest con was that the readlength(for now) is only 50bp and PE is not possible yet. But they aim at reading the other strand instead of reading the same strand backwards, meaning they can achieve same read length reverse as forward in the same quality at the same speed.

Last edited by Zaag; 07-24-2012 at 10:35 AM.
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Old 11-07-2012, 05:11 AM   #11
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Anyone taken the plunge and upgraded to Wildfire? We likely to be doing it in the next few months. PE 50bp by years end apparently.
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Old 11-13-2012, 12:45 PM   #12
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We have one now, I'll be running an old sample this week. I will post the results.
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Old 12-03-2012, 09:09 AM   #13
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lane1: 240 million 0 mismatch barcode reads; 80% aligned to reference (hg19)
lane2: 200 million 0 mismatch barcode reads, 71% aligned to reference (hg19)

both lanes contained the same sample, only the amount we loaded at the slides to form colonies was higher in lane2
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Old 12-04-2012, 02:58 AM   #14
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How many raw reads did you get per lane?
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Old 12-04-2012, 06:15 AM   #15
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Default Library/adapter compatibility

Can anyone tell me if Wildfire will work with standard SOLiD libraries, or even better, what the Wildfire adapter sequences are?
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Old 12-05-2012, 04:33 AM   #16
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Quote:
Originally Posted by Chipper View Post
How many raw reads did you get per lane?
450 million and 400 million Total reads from the multiplex report. I think this can be higher if we find the optimal amount to load on the slide.
Quote:
Originally Posted by SeqTruth View Post
Can anyone tell me if Wildfire will work with standard SOLiD libraries, or even better, what the Wildfire adapter sequences are?
You can PCR the Wildfire adapters to a 5500 library so that you can run it on the wildfire. I don't know the sequence.
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Old 01-07-2013, 09:48 AM   #17
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Hi,

I will be in AGBT looking at this from a different angle. Perhaps someone has link/more information please.

I run a genomics facility for CR-UK and we have GA's, HiSeq and an Ion torrent to do validation sequencing and for that it's a good little tool. The down fall is the emulation PCR. Now when something goes wrong it is pretty much back to that. Itís expensive and time consuming.

Now, if you haven't worked out what I want to do yet, I want to use the wildfire library production and its non-emulation PCR and run on my Ion torrent but I can't find any details. As academia I do not have to worry about the issues companies have with IP. So, I want to give it a go. Can anyone send me links to papers, information or help on this I will buy you a drink as AGBT.

All the best

Nik
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Old 01-21-2013, 05:40 AM   #18
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This doesn't sound practical to me, the amplification of the WF protocol happens on the slide (as with the Illumina range) so it won't be portable to the ion chip....could you imagine using the GA protocol to make libraries for the Ion?

My understanding is that there will be an emulsionPCR free strategy on the ion, they are calling 'Avalanche' but I don't think there is a release data yet.
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Old 03-27-2013, 04:31 AM   #19
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Hi, I need your help please.

I accidentaly ordered run cycle buffers and buffer D for solid wildfire, although we do have 5500xl only. Do you think i can use these buffers, or throw them away?
thanks.
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Old 03-27-2013, 03:02 PM   #20
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Quote:
Originally Posted by Brunda View Post
Hi, I need your help please.

I accidentaly ordered run cycle buffers and buffer D for solid wildfire, although we do have 5500xl only. Do you think i can use these buffers, or throw them away?
thanks.

The actual sequencing on Wildfire is chemically the same as 5500xl provided they haven't moved the buffer locations on deck (that's easy to check).
It should work.
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