SEQanswers

Go Back   SEQanswers > Bioinformatics > Bioinformatics



Similar Threads
Thread Thread Starter Forum Replies Last Post
Primer sequences emPCR and sequencing primer Ion Torrent laurakrol Sample Prep / Library Generation 1 04-25-2014 04:08 AM
new to bio: Can primer walking only be used in Shotgun sequencing? arkilis Bioinformatics 4 07-29-2013 11:32 PM

Reply
 
Thread Tools
Old 02-02-2016, 08:42 AM   #1
simon_seq
Member
 
Location: Cambridge, UK

Join Date: Aug 2012
Posts: 13
Default Automated primer walking

Hi all,
I'm trying to generate a list of oligonucleotide primers for Sanger sequencing, such that for an arbitrarily large DNA sequence I would get an optimised oligonucleotide every ca. 500 bases. How is this best possible? Solutions I have thought along are:
- BatchPrimer 3 (http://probes.pw.usda.gov/batchprimer3/)
- Querying NCBI Primer-Blast through R => but is there a tool for that..?

Thanks!
Simon
simon_seq is offline   Reply With Quote
Reply

Tags
pcr, primer, sanger sequencing

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:58 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO