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  • multiplex few variants in hundreds of samples

    Dear all,

    I would like to know if someone is working (or knows someone that is working) on a method to be able to multiplex sequencing of very few variants (let say 100 SNPs) on hundreds or maybe thousands of samples in a single HiSeq run. There is a market need for this kind of product but as far as I know, no solutions are yet available.

  • #2
    Would this be appropriate? http://www.illumina.com/technology/g...ing_assay.ilmn

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    • #3
      Originally posted by fadista View Post
      Dear all,

      I would like to know if someone is working (or knows someone that is working) on a method to be able to multiplex sequencing of very few variants (let say 100 SNPs) on hundreds or maybe thousands of samples in a single HiSeq run. There is a market need for this kind of product but as far as I know, no solutions are yet available.
      Already answered elsewhere on the forums:

      We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of s …

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