Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • An error about annotation exon number of gene in tophat-fusion report

    I found an error about annotation gene.

    In my result, one candidates report by tophat-fusion as follwing:

    T2852 chr15-chr12 62173825 6979426 ff
    6 1 6 2 35 41
    TTACTGTACTCATTAAATCTTGTGATGACACTCACATCAATGAAAGGCTT AGGCTCTGTGACTGGGGCAACCTGCAAGGAGCTGGCCAGCCAGCCTGATG
    00000000000000066666666666666666666666666666666666 66666666666666666666666666666666666666666000000000
    VPS13C exon15(62173760-62173830) TPI1 exon7(6979427-6980109)

    The error is that VPS13C express from genome revere site, the exon number is 71 instead of 15 (total exon number is 86).

    exon number in genome:86<-85<-84.....71<--- (true)

    exon number in genome:1->2->3->.....->15-> (error)

  • #2
    messed up exon information from tophat-fusion

    I also experienced similar problem with well known fusion.

    Expected fusion in my sample is TMPRSS2(Exon1) with ERG. What I obtain is TMPRSS2 (exon14) with ERG which doesn't make sense.

    Is there a solution to this problem? Please suggest how to get the right fusions from tophat-fusion?

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 11:49 AM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 08:47 AM
    0 responses
    16 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    61 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Working...
    X