Hi,
I don't know a lot about sequencing, my question should be trivial but I cannot find the answer on the web...
Suppose that a genome contains a certain sequence, like AGTACATGG.
My question is: if I sequence this genome, in how many possible ways can this sequence be represented in my data? Does it depend on the sequencing platform?
Here is the list of all the potential possibilities, as far as I can think:
1: "original"
AGTACATGG
2: reverse complement
CCATGTACT
3: complement
TCATGTACC
4: reverse
GGTACATGT
Thank you very much for your help!
I don't know a lot about sequencing, my question should be trivial but I cannot find the answer on the web...
Suppose that a genome contains a certain sequence, like AGTACATGG.
My question is: if I sequence this genome, in how many possible ways can this sequence be represented in my data? Does it depend on the sequencing platform?
Here is the list of all the potential possibilities, as far as I can think:
1: "original"
AGTACATGG
2: reverse complement
CCATGTACT
3: complement
TCATGTACC
4: reverse
GGTACATGT
Thank you very much for your help!
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