Hello,
My miseq showed an error and stoped running two hours after the run started.
Error: Through- focus peak did not exceed SNR threshold (Illumina.Instrument.Bolt.Logic.AutoFocus.DarkSampleException)</ErrorDescription>
It was using a V2X 500cycles kit with 24 samples from SOPHIA Genetics NGS Panel.
Could you explain me which could be the reason and what could be the solution?
My miseq showed an error and stoped running two hours after the run started.
Error: Through- focus peak did not exceed SNR threshold (Illumina.Instrument.Bolt.Logic.AutoFocus.DarkSampleException)</ErrorDescription>
It was using a V2X 500cycles kit with 24 samples from SOPHIA Genetics NGS Panel.
Could you explain me which could be the reason and what could be the solution?
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