Hello,
I currently have illumina sequencing data and already done denovo assembly and the stuffs needed for that project.
Now, I am interested in restriction enzyme digestion followed by sequencing. But before running that project I would like to see how it would look like. So, basically I want to cut already sequenced sequences with some restriction enzyme in silico. I don't want to cut just the consensus sequence but all of those sequences and assemble again and see if those cut sequences assemble to form the same consensus sequence an so forth. I may also want to introduce some randomness if possible like missing some true sites.
Is there some program to do this task. or part of it?
Thank you
I currently have illumina sequencing data and already done denovo assembly and the stuffs needed for that project.
Now, I am interested in restriction enzyme digestion followed by sequencing. But before running that project I would like to see how it would look like. So, basically I want to cut already sequenced sequences with some restriction enzyme in silico. I don't want to cut just the consensus sequence but all of those sequences and assemble again and see if those cut sequences assemble to form the same consensus sequence an so forth. I may also want to introduce some randomness if possible like missing some true sites.
Is there some program to do this task. or part of it?
Thank you
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