De Novo assembly

[G_mich]

Hello,

I want to do a De Novo assembly of a select region of interest for a library of paired end reads sequenced using Illumina HiSeq. I will appreciate any suggestion for a downloadable tool.

Thanks!

[sivasubramani]

fermi, minia.. these will work fast also takes less memory.. Velvet, SOAP denovo are like super stars in assembly, MIRA would be better if you have sooooo much patiance...

Good luck

[winsettz]

Hello,

I want to do a De Novo assembly of a select region of interest for a library of paired end reads sequenced using Illumina HiSeq. I will appreciate any suggestion for a downloadable tool.

Thanks!

Organism? Size of region of interest?

[G_mich]

fermi, minia.. these will work fast also takes less memory.. Velvet, SOAP denovo are like super stars in assembly, MIRA would be better if you have sooooo much patiance...

Good luck

Thank you!

[G_mich]

Organism? Size of region of interest?

Human! 800-1000 bp.