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Old 08-13-2010, 12:26 PM   #1
gfmgfm
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Default wig files

Hello,
I am new in the field of NGS. I did a small RNA SEQ experiment. I have a blast output of tags and I want to create wig files for IGV. I looked for instructions on creating such files. Does anyone has a link to such instructions or can give any tips?
Thanks.
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Old 08-14-2010, 12:32 AM   #2
dawe
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Hi, you can find wig specs here. After that just load it into IGV

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Old 08-14-2010, 07:09 PM   #3
adamdeluca
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You can also create a bed graph file

Chr start stop value

And convert it to wig/bigwig with the utilities provided by ucsc
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Old 08-14-2010, 09:45 PM   #4
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Thanks a lot.
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