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Old 09-20-2011, 06:23 AM   #1
jlewi
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Location: United States

Join Date: Sep 2011
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Default Canonical direction and assembly

Hi,

I'm looking at Contrail and trying to understand what "Canonical Sequence Direction" means. In Contrail the canonical sequence direction depends on whether the sequence or its reverse complement comes first lexicographically. I assume canonical direction is somehow used to consider the fact that the read might have come from either strand. Can anyone shed more light on this?

Thanks
Jeremy
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