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Old 08-10-2009, 03:20 AM   #1
Will
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Location: London

Join Date: Aug 2009
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Lightbulb Illumina Primers

Hi everyone,

I am new to the SEQanswers community and have been searching through
the threads to find primer and adapter concentrations for use with the Illumina GA II system.

I am planning to do MeDIP-Seq for Paired-end multiplexed (index/tagged) samples.

In some of the other threads it is implied that the sequencing primers are 100um. However does this concentration apply to the adapters or the primers for LM-PCR ??

Also another thing has me slightly confused.
Some groups achieve multiplexing by designing tagged adapters, whereas others add the tag during LM-PCR. Is this because it's not too important when the tag goes in or are there specific advantages to either strategy.

Considered thoughts would be greatly appreciated.

Cheers

Will
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Old 08-10-2009, 08:08 AM   #2
Will
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Location: London

Join Date: Aug 2009
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Never mind !

I have been shown the error of my ways
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