We are using the Chemagen system (http://www.chemagen.com/fileadmin/do...n_brochure.pdf) to extract total nucleic acid from blood and stool samples. The process yields a mixture of purified DNA/RNA, but doesn't suggest how to separate the two. Has anyone done this type of separation before? I know Qiagen uses columns in their AllPrep system, but they can't be purchased separately. Most RNAse or DNase protocols I see are designed to remove small amounts of contaminating RNA/DNA, I don't know how they'd do with total nucleic acid?
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by seqadmin
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
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05-06-2024, 07:48 AM -
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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