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I have exosome rna-seq data with ~13M reads. The data is 50bp single-end from HiSeq 2000 machine. Nugen ovation kit v2 was used for library preparation from total RNA. I aligned the reads to the human genome after quality trimming and got <1% of the reads aligned to the genome. I checked statistics on the sequenced reads and noticed that 90% of the reads were duplicated. Could this be the issue? I appreciate your insights on what might be the issue.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM