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Old 02-22-2018, 11:39 AM   #1
thiNGS
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Default Nexseq run - very low density but good intensity

Hi all. I am running some samples on the nextseq and I have seen that after cycle 5 the intensity drops dramatically. At cycle 1 intensity was alright but then it massively goes down. The cluster density is also very low although I have followed the protocol properly and diluted my library to 1.8 pM. Is there a way I can check on basespace if this is due to a faulty flow cell?
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Old 02-22-2018, 12:51 PM   #2
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Update: I can't see any phix data either although the library has been spiked in with a phix I used last week. It seems like phix has not been sequenced at all. Any thoughts? I am really confused, this library passed all the QCs...
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