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  • RNA editing softwares

    Hi ,

    I am a newbie to this field , I am currently trying to work on finding rna editing sites , are there any well known softwares and papers which deal with this issue?

    I currently know of rddChecker and expedit , but are there any other softwares apart from these , also if possible can someone point me out to some good links to tutorials on rna editing so that I ca understand the subject matter better

  • #2
    Hi,
    There are plenty of papers dealing with RNA editing at the PubMed. For example:
    RNA-seq data can be mined for sequence differences relative to the reference genome to identify both genomic SNPs and RNA editing events. We analyzed the long, polyA-selected, unstranded, deeply sequenced RNA-seq data from the ENCODE Project across 14 human cell lines for candidate RNA editing event …

    We show that despite over two million years of evolutionary divergence, the sites edited and the level of editing at each site is remarkably consistent across the 15 strains. In the Cds2 gene we find evidence for RNA editing acting to preserve the ancestral transcript sequence despite genomic sequen …

    They deploy interesting analysis pipelines.
    Expedit can be useful if you work with human genomes. I haven't test rddChecker. Other options: VarScan and Samtools-bcftools. They have a slightly different variant calling approach.

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    • #3
      Thank you for the reply . I am looking into it . Also do the software's listed consider both mis-aligment and multiple alignment issue's.?

      Comment

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