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Old 05-05-2019, 03:18 PM   #1
cement_head
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Location: Oxford, Ohio

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Default MiSeq transcriptome [load]

Hi,

We're doing a quick & dirty transcriptome for a low-complexity tissue using a single library on a MiSeq; time/cost are a factor. The run is a single sample RNA-Seq; V2 reagent; 2x150 PE. Illumina recommends 12.5 pM for their PhiX control, but in the interest of not overloading the run, I'm thinking of about a 10 pM load with a 2% PhiX (@ 10 pM stock) spike-in. Would I be pushing the upper limit - the library fragment size (by KAKA) is 470 bp. I don't normally do a transcriptomes/RNA-Seq on the MiSeq, and thus the question. Thoughts?

Thanks...
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