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Old 05-08-2019, 10:08 PM   #1
sidolfus
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Default Increasing the number of samples of NextSeq 500 v2.5 high output flow cell

Is it possible to increase the number of samples of NextSeq 500 v2.5 high output flow cell beyond the recommended amount of 12 samples for exome sequencing? Has it been done before?
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Old 05-09-2019, 02:56 AM   #2
GenoMax
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As long as you have a unique set of index sequences it is certainly possible. The total yield from a FC is not going to change so that final read number will get divided into the samples you put in the pool i.e. each sample will get proportionately less number of reads.
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Old 05-11-2019, 08:35 AM   #3
sidolfus
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Thank you for your reply GenoMax
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