Tweet
I have a control and a knockdown experiment from which I am sequencing small rnas. When I count the sum of the reads mapped to the known mirnas foreach condition I get 968384 for contol anD 71736 for the knockdown. I really dont know how to interpret the results and if normalizing by the total nr of reads makes sence. Important I cannot use the assumption that the expression of most miRNAs should not change. Any suggestions?
-
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM