Hi everyone,
I want to use dwgsim to simulate solid reads for testing SOLiD denovo2 pipeline(which needs a F3 and another R3 or F5). But I'm little confused with the read orientation of "out.bfast.fq".
Eg, first two reads from my out.bfast.fq is here,
@I_532_32_1_1_0:0:0_0:0:0_0
A2133112101103112111031211
+
1111111111111111111111111
@I_532_32_1_1_0:0:0_0:0:0_0
A3130100331131313012113102
+
1111111111111111111111111
According to dwgsim wiki, the 4th the and 5th field of the read name describing the strand
4. strand end 1 (0 - forward, 1 - reverse)
5. strand end 2 (0 - forward, 1 - reverse)
so my question id how to separate these reads to corresponding F3 and R3(or F5) files ?
Is that first one is F3 and second is R3 ?
I want to use dwgsim to simulate solid reads for testing SOLiD denovo2 pipeline(which needs a F3 and another R3 or F5). But I'm little confused with the read orientation of "out.bfast.fq".
Eg, first two reads from my out.bfast.fq is here,
@I_532_32_1_1_0:0:0_0:0:0_0
A2133112101103112111031211
+
1111111111111111111111111
@I_532_32_1_1_0:0:0_0:0:0_0
A3130100331131313012113102
+
1111111111111111111111111
According to dwgsim wiki, the 4th the and 5th field of the read name describing the strand
4. strand end 1 (0 - forward, 1 - reverse)
5. strand end 2 (0 - forward, 1 - reverse)
so my question id how to separate these reads to corresponding F3 and R3(or F5) files ?
Is that first one is F3 and second is R3 ?
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