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Old 08-26-2011, 03:01 AM   #1
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Default PubMed: Effect of Sample Storage Conditions on Culture-independent Bacterial Communit

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Effect of Sample Storage Conditions on Culture-independent Bacterial Community measures in Cystic Fibrosis Sputum Specimens.

J Clin Microbiol. 2011 Aug 24;

Authors: Zhao J, Li J, Schloss PD, Kalikin LM, Raymond TA, Petrosino JF, Young VB, Lipuma JJ

Abstract
Culture-independent approaches to studying the microbiota of the airways of cystic fibrosis (CF) patients have employed analysis of the bacterial 16S rRNA gene through Sanger sequencing of clone libraries (5, 6, 12), terminal restriction fragment length polymorphism analysis (8, 11), microarray hybridization (3, 7), or pyrosequencing (2, 5) and have revealed the presence of far more complex bacterial communities than has been appreciated with culture-based methods.


PMID: 21865433 [PubMed - as supplied by publisher]



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