SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Help with TruSeq gDNA library prep BTS Illumina/Solexa 45 02-22-2017 11:09 AM
TruSeq or Nextera kit for library prep? rooty Sample Prep / Library Generation 12 10-29-2013 07:02 AM
Automation for TruSeq library prep? jlove Sample Prep / Library Generation 10 01-10-2012 09:34 AM
Truseq RNA library has 2 peak on bioanalyzer houda Sample Prep / Library Generation 7 07-06-2011 11:39 AM
Fragment Size shift on Egel bye using the new TruSeq protocol ? Claire1 Sample Prep / Library Generation 2 05-05-2011 01:37 PM

Reply
 
Thread Tools
Old 11-14-2011, 03:37 AM   #1
HGENETIC
still a novice
 
Location: Cardiff

Join Date: Jul 2010
Posts: 34
Default TruSeq library size

Hi all,

I'm setting up a library prep using TruSeq DNA sample enrichment kits, I've sheared the DNA using Covaris and have run the product on a bioanalyzer. My library peaks seem to be around the 55-0600bp mark whereas previously I would get them nearer 300bp. I'm planning to sequence on an illumiina GA11x with 110bp read length, does anyone one have an idea if these fragments need to be sheared again to make them smaller or are they OK?

Thanks in advance for any help - H
HGENETIC is offline   Reply With Quote
Old 11-14-2011, 03:46 AM   #2
nickloman
Senior Member
 
Location: Birmingham, UK

Join Date: Jul 2009
Posts: 356
Default

Should be OK. And as a bonus, if you sequence in both directions you'll get a nice long insert which should help with mapping or assembly.
nickloman is offline   Reply With Quote
Old 11-14-2011, 03:56 AM   #3
HGENETIC
still a novice
 
Location: Cardiff

Join Date: Jul 2010
Posts: 34
Default

Quote:
Originally Posted by nickloman View Post
Should be OK. And as a bonus, if you sequence in both directions you'll get a nice long insert which should help with mapping or assembly.
Thanks nickloman, that's a relief!!
HGENETIC is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 08:27 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO