Go Back   SEQanswers > Literature Watch

Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Quantitative analysis of DNA methylation of imprinted genes in single human b Newsbot! Literature Watch 0 05-18-2011 03:00 AM
PubMed: Quantitative analysis of DNA methylation at all human imprinted regions revea Newsbot! Literature Watch 0 02-02-2011 03:00 AM
PubMed: Profiling DNA methylation from small amounts of genomic DNA starting material Newsbot! Literature Watch 0 11-07-2008 10:30 AM
PubMed: Genome-wide high throughput analysis of DNA methylation in eukaryotes. Newsbot! Literature Watch 0 10-28-2008 06:00 AM

Thread Tools
Old 05-22-2009, 06:00 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default PubMed: Quantitative analysis of DNA methylation after whole bisulfitome amplificatio

Syndicated from PubMed RSS Feeds

Related Articles Quantitative analysis of DNA methylation after whole bisulfitome amplification of a minute amount of DNA from body fluids.

Epigenetics. 2009 May 24;4(4)

Authors: Vaissière T, Cuenin C, Paliwal A, Vineis P, Hoek G, Krzyzanowski M, Airoldi L, Dunning A, Garte S, Hainaut P, Malaveille C, Overvad K, Clavel-Chapelon F, Linseisen J, Boeing H, Trichopoulou A, Trichopoulos D, Kaladidi A, Palli D, Krogh V, Tumino R, Panico S, Bueno-De-Mesquita H, Peeters P, Kumle M, Gonzalez C, Martinez C, Dorronsoro M, Barricarte A, Navarro C, Quiros J, Berglund G, Janzon L, Jarvholm B, Day N, Key T, Saracci R, Kaaks R, Riboli E, Hainaut P, Herceg Z

Cell-free circulating DNA isolated from the plasma of individuals with cancer has been shown to harbor cancer-associated changes in DNA methylation, and thus it represents an attractive target for biomarker discovery. However, the reliable detection of DNA methylation changes in body fluids has proven to be technically challenging. Here we describe a novel combination of methods that allows quantitative and sensitive detection of DNA methylation in minute amounts of DNA present in body fluids (quantitative Methylation Analysis of Minute DNA amounts after whole Bisulfitome Amplification, qMAMBA). This method involves genome-wide amplification of bisulphite-modified DNA template followed by quantitative methylation detection using pyrosequencing and allows analysis of multiple genes from a small amount of starting DNA. To validate our method we used qMAMBA assays for four genes and LINE1 repetitive sequences combined with plasma DNA samples as a model system. qMAMBA offered high efficacy in the analysis of methylation levels and patterns in plasma samples with extremely small amounts of DNA and low concentrations of methylated alleles. Therefore, qMAMBA will facilitate methylation studies aiming to discover epigenetic biomarkers, and should prove particularly valuable in profiling a large sample series of body fluids from molecular epidemiology studies as well as in tracking disease in early diagnostics.

PMID: 19458486 [PubMed - as supplied by publisher]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 09:52 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO