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Old 06-26-2009, 06:27 AM   #1
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Location: Germany

Join Date: May 2009
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Default 3'UTR library or random primed cDNA library for quantification?

I am quite new in the field of next generation sequencing and I hope to get some information and help here.
I want to do a RNA-Seq by using the Illumina platform to analyze the transcript profile of a specific tissue of a wildtype plant compared to a mutant. The aim is identifying a different transcription level of some genes as well as finding some rare genes. The organism I am working with is not sequenced yet, but it should be till the end of this year.
My question is which type of library is more suitable for quantification, a random primed cDNA library or a 3’UTR library, which proposed me a company. But what would be the advantage of a 3’UTR library?

Thank you very much!
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