SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Library on gel but not on Bioanalyzer pauji Sample Prep / Library Generation 6 05-13-2012 06:30 PM
weird gel-size selection of 454 library havana Sample Prep / Library Generation 0 03-22-2012 01:29 AM
library gel purification with SYBR gold problems shawpa Sample Prep / Library Generation 3 01-16-2012 01:25 PM
New, Completely Gel-Free, DNA Library Prep Protocol Bioo Scientific Vendor Forum 1 01-11-2012 08:49 AM
E-Gel SizeSelect? Sandokhan Sample Prep / Library Generation 0 10-27-2011 08:01 AM

Reply
 
Thread Tools
Old 09-06-2012, 06:37 PM   #1
Mollycule
Junior Member
 
Location: Ithaca, NY

Join Date: Sep 2012
Posts: 1
Default Can't see my library on a gel

Hi everyone, sequencing newbie here!

I'm finishing up my first Illumina RNA-seq library prep, and my protocol suggests running my library out on a gel to check for a smear of the appropriate size (~300 bp). But when I do this, I don't see my library on the gel--even after 18 cycles of amplification, and even with a sensitive stain like SYBR Gold. Well, I do see faint adapter dimers, but I suspect these can be removed with Ampure XP cleanup. Does this mean my library yield is too low to proceed with sequencing? What is an acceptable yield range, and how do you guys do your library QC?

Any input is appreciated. Thanks!
Mollycule is offline   Reply With Quote
Old 09-06-2012, 09:23 PM   #2
jinpingzhang
Member
 
Location: Houston

Join Date: Mar 2012
Posts: 10
Default

Hi,
I am not sure Illumina RNA seq library length and concentration. but i am sure the SOLiD RNA library some information. After your PCR and purification process, you will get several ng library, use Agilent High Sensitive DNA Chip check them.
jinpingzhang is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 06:47 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO