Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
DEXSeq error in estimateDispersions: match.arg(start.method, c("log(y)", "mean")) fpadilla Bioinformatics 14 07-03-2013 03:11 PM
Are there tools for "de novo" assembly of rna-Seq and genomic DNA rexxi Bioinformatics 1 08-31-2012 12:16 PM
strategy to convert low GQ calls to "no call" manducasexta Bioinformatics 0 08-27-2012 09:49 AM
usearch --cluster causes "Segmentation fault (core dumped)" elfuser Bioinformatics 1 07-02-2012 12:42 AM
Samtools problem on a "rocks" cluster GenoMax Bioinformatics 8 08-24-2009 05:15 AM

Thread Tools
Old 01-07-2013, 04:19 AM   #1
Junior Member
Location: Cambridge, UK

Join Date: Dec 2011
Posts: 3
Question Low cluster density of Truseq "high throughput" libraries - both DNA and RNA

Hi genomics folks,

Has anyone got any experience in clustering Illumina Truseq RNA 'HT' (high throughput) and Truseq DNA HT libraries?

I work in an NGS service, and we recently tried out the new Truseq HT kits. I like the idea of multiplexing up to 96 Truseq libraries within a single lane.

These libraries were quantified by qPCR following our normal protocol for Truseq libraries, using the KAPA library quant kit. They were then clustered using a cbot -> HiSeq or straight on to the MiSeq. The resulting cluster density was 1/4 to 1/2 of what we would expect, on both machines.

These projects were unrelated and the library prep was performed by very experienced guys in the lab - I'm starting to wonder if the HT kits require different clustering conditions. According to Illumina, these libraries should cluster OK using the normal protocol.

Has anyone else experienced this problem?
SLeigh-Brown is offline   Reply With Quote

cluster density, library quantification, truseq high throughput

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:45 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO