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Old 09-27-2018, 06:03 AM   #1
Location: US

Join Date: Jan 2016
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Default BluePippin use w/ Nextera Kits

Always used BluePippin with XT kit, as a two-step size selection. Trying out the new Flex kit. Since the protocol calls for a set/given amount of beads in the Flex kit, will the BluePippin size selection be overkill? Has anyone used the two in combination? Does it work better or is the Flex kit best used alone? Thanks.
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Old 09-28-2018, 05:10 AM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,248

Nextera DNA Flex workflow includes a double SPRI size selection. A Pippin size selection will reduce library diversity and is not required unless you have a specific application that needs tight size selection.
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