Hi there,
If you have situation that you have files from three sequencing platforms ( DNA-Seq / RNA-Seq and exome sequencing ) and do not know which belongs to which, can you distinguish them in some way?
I have an idea, but wanted to check what you guys think?
- Map some reads from each technology to one chromosome ( to save time and computing power )
- The reads that map everywhere, exons + introns = DNA-Seq reads
- Reads that map to exons +- 50bp = Exome seq reads
- Reads which have very less or zero coverage in intronic regions = RNA-Seq reads.
What do you guys think? Is there any other way? ( Other than files sizes )
Thanks a ton,
If you have situation that you have files from three sequencing platforms ( DNA-Seq / RNA-Seq and exome sequencing ) and do not know which belongs to which, can you distinguish them in some way?
I have an idea, but wanted to check what you guys think?
- Map some reads from each technology to one chromosome ( to save time and computing power )
- The reads that map everywhere, exons + introns = DNA-Seq reads
- Reads that map to exons +- 50bp = Exome seq reads
- Reads which have very less or zero coverage in intronic regions = RNA-Seq reads.
What do you guys think? Is there any other way? ( Other than files sizes )
Thanks a ton,
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