I've been having horrible over-clustering issues with 16S and RNA-Seq (microbial) runs on our MiSeq.
The most recent was a 16S amplicon run (600 bp) and a 3.5 pM library + (25% spike-in) 12.5 pM PhiX on a 300v2 kit. Got cluster density of >1400 K/mm2.
This has never happenend at this [library] for 16S. Should I go as low as 1.8 pM ~ 2 pM?
The most recent was a 16S amplicon run (600 bp) and a 3.5 pM library + (25% spike-in) 12.5 pM PhiX on a 300v2 kit. Got cluster density of >1400 K/mm2.
This has never happenend at this [library] for 16S. Should I go as low as 1.8 pM ~ 2 pM?
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