We have a short reads problem when sequencing previously successfully sequenced library. Could somebody help me to figure out our problem?
Img1.png represent our first sequencing run of this library. Then at the same time we prepared two LV emPCR for this library. Img2.png represent our second sequencing run using beads from one LV emPCR preparation above and img3.png - third sequencing run using beads from another LV emPCR preparation.
Where these huge number of small fragments came from? 99% of reads carry same adapters of this library, so its not some sort of contamination.
Thanks!
Img1.png represent our first sequencing run of this library. Then at the same time we prepared two LV emPCR for this library. Img2.png represent our second sequencing run using beads from one LV emPCR preparation above and img3.png - third sequencing run using beads from another LV emPCR preparation.
Where these huge number of small fragments came from? 99% of reads carry same adapters of this library, so its not some sort of contamination.
Thanks!
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