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Old 10-07-2018, 11:09 PM   #1
sage12
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Location: USA

Join Date: Aug 2018
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Default Limma multifactor experiment design matrix

Hi, so I am very confused as to how I can make a multifactor design for limma RNA differential expression. I've read other posts in different forums and none of the answers are very explicit (to me) as to how to build a multifactor design

For examples, say I have p1N, p1T, p2N, and p2T, where p# is patient number and N/T is normal tumor.

I can build a single factor design matrix like this:

------Normal------Tumor
1 ------ 1 --------- 0
2 ------ 0 --------- 1
3 ------ 1 --------- 0
4 ------ 0 --------- 1

I am completely lost as to how I can build a numeric matrix with multiple factors, which in this case, the factors would be patient number and N/T.
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