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Old 12-18-2018, 07:47 AM   #1
richard.nadvornik
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Location: Czech Republic

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Default Next-Generation Sequencing data analysis – Workshop for beginners, May 22-24 2019

Brno, Czech Republic, See here for full event details and apply online

Nowadays, Next-Generation sequencing technologies form an essential part of almost all disciplines of biological sciences in research and diagnostics. Due to their quantity and namely complexity, NGS data are typically processed by qualified bioinformaticians or computational biologists. This presents a serious issue even for experienced researchers who have never studied this field of science simply because it has emerged just recently and suddenly there is a need to adapt to completely new tools, workflows and scientific terminology not to perform this sequencing data analysis process – after all, this is why we employ bioinformaticians, but to understand it properly.

Our workshop gives you the opportunity to understand basics of NGS data processing. The aim of the workshop is to teach you perform basic steps of NGS data analysis including but not limited to raw data manipulations, quality control, removal of low-quality sequences, sequencing adapters, artifacts, or other contaminations, and especially preparation of a high-quality data set that can be used for downstream analysis.

You will get to know various NGS data formats, learn about specific problems of sequencing technologies used and how to handle them, and understand the background of individual processes in NGS data editing. You will not become a bioinformatician after this 3-day workshop, but you will set off for becoming a qualified partner for bioinformaticians in your team.

Who should attend?
This workshop is designed for biologists or beginners in bioinformatics who perform or plan to perform Next-Generation sequencing data analysis. No prior knowledge of sequence data analysis or specific programming skills are required.

During the workshop…
  • Trainers will provide lectures with follow-up hands-on exercises in order to have the participants learning and assessing their knowledge
  • All workshop participants will perform all steps of data analysis tasks themselves!
  • The powerful computer infrastructure available at the conference center will be used (no personal laptops are required/allowed)

Workshop agenda

Day 1: Unix and how do I use it? - Get to know the environment
  • General introduction to NGS data
  • Overview of NGS data formats
  • Introduction to the Linux system
  • Navigation and basic commands in the Terminal

Day 2: NGS data quality control - Was my sequencing successfull?
  • Standard commands in the Terminal to process NGS data
  • Visualization of the quality of raw NGS data
  • Pre-processing of short-reads FASTA/FASTQ files using various programs

Day 3: How do I get high quality NGS data set? - Get the best out of it!
  • Read mapping to the reference sequence
  • Visualization and evaluation of mapping quality
  • Identification and removal of duplicate reads
  • Identification of unmapped reads – Blast
  • ...

See here for full event details and apply online
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